Characterization of chitinase isoforms from grape juice

  • D. Gazzola Università di Padova
  • G. Pasini Università di Padova
  • S. Tolin Università di Padova
  • A. Curioni Università di Padova
  • S. Vincenzi Università di Padova

Abstract

Grape chitinases are recognized as being mainly responsible for protein haze formation in white wines. Vitis vinifera L. cv. Manzoni Bianco grape juice proteins were fractionated using anion exchange and hydrophobic interaction chromatographies. According to SDS-PAGE and zymography, six protein bands with chitinolytic activity were subjected to mass spectrometry (MALDI-TOF/TOF MS), which assigned all the bands to Vitis vinifera class IV chitinases. These grape chitinase isoforms showing different electrophoretic and chromatographic behaviors are likely to be also distinct in their functionality in wine. This could be relevant to understand the involvement of single chitinase components in wine hazing and to develop specific winemaking techniques for their removal from wine.

Author Biographies

D. Gazzola, Università di Padova
Department of Agronomy, Food, Natural Resources, Animals and Environment
G. Pasini, Università di Padova
Department of Agronomy, Food, Natural Resources, Animals and Environment
S. Tolin, Università di Padova
Proteomics Center
A. Curioni, Università di Padova
Department of Agronomy, Food, Natural Resources, Animals and Environment

References

Collinge D.B., Kragh K.M., Mikkelsen J.D., Nielsen K.K., Rasmussen U. and Vad K. 1993. Plant chitinases. Plant J. 3 (1): 31.
Dahiya N., Tewari R. and Hoondal G.S. 2006. Biotechnological aspects of chitinolytic enzymes: a review. Appl. Microbiol. Biot. 71 (6): 773.
Dambrouck T., Marchal R., Cilindre C., Parmentier M. and Jeandet P. 2005. Determination of the grape invertase content (using PTA-ELISA) following various fining treatments versus changes in the total protein content of wine. Relationships with wine foamability. J. Agric. Food Chem. 53 (22): 8782.
Dorrestein E., Ferreira R.B., Laureano O. and Teixeira A.R. 1995. Electrophoretic and FPLC analysis of soluble proteins in four Portuguese wines. Am. J. Enol. Viticult. 46 (2): 235.
Dufrechou M., Vernhet A., Roblin P., Sauvage F. X. and Poncet-Legrand C. 2013. White Wine Proteins: How does the pH affect their conformation at room temperature?. Langmuir. 29 (33): 10475.
Dufrechou M., Poncet-Legrand C., Sauvage F. X. and Vernhet A. 2012. Stability of White Wine Proteins: Combined Effect of pH, Ionic Strength, and Temperature on Their Aggregation. J. Agric. Food Chem. 60 (5): 1308.
Esteruelas M., Poinsaut P., Sieczkowski N., Manteau S., Fort M.F., Canals J.M. and Zamora F. 2009. Characterization of natural haze protein in sauvignon white wine. Food Chem. 113 (1): 28.
Falconer R.J., Marangon M., Van Sluyter S.C., Neilson K.A., Chan C. and Waters E.J. 2010. Thermal stability of thaumatin-like protein, chitinase, and invertase isolated from Sauvignon blanc and Semillon juice and their role in haze formation in wine. J. Agric. Food Chem. 58 (2): 975.
Ferreira R.B., Picarra-Pereira M.A., Monteiro S., Loureiro V.B. and Teixeira A.R. 2001. The wine proteins. Trends Food Sci Tech. 12: 230.
Gazzola D., Van Sluyter S.C., Curioni A., Waters E.J. and Marangon M. 2012. Roles of proteins, polysaccharides, and phenolis in haze formation in white wine via reconstitution experiments. J. Agric. Food Chem. 60 (42): 10666.
Gazzola D., Vincenzi S., Pasini G., Lomolino G. and Curioni A. 2015. Advantages of the KDS/BCA assay over the Bradford assay for protein quantification in white wine and grape juice. Am. J. Enol. Viticult. 66: 227.
Graham L.S. and Sticklen M.B. 1994. Plant chitinases. Can. J. Botany. 72: 1057.
Laemmli U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature. 227 (5259): 680.
Manteau S., Lambert B., Jeandet P. and Legendre L. 2003. Changes in chitinase and thaumatin-like pathogenesis-related proteins of grape berries during the Champagne winemaking process. Am. J. Enol. Viticult. 54: 267.
Marangon M., Van Sluyter S.C., Haynes P.A. and Waters E.J. 2009. Grape and wine proteins: their fractionation by hydrophobic interaction chromatography and identification by chromatographic and proteomic analysis. J. Agric. Food Chem. 57 (10): 4415.
Marangon M., Vincenzi S., Lucchetta M. and Curioni A. 2010. Heating and reduction affect the reaction with tannins of wine protein fractions differing in hydrophobicity. Anal. Chim. Acta. 660: 110.
Marangon M., Van Sluyter S.C., Neilson K.A., Chan C., Haynes P.A., Waters E.J. and Falconer R.J. 2011. Roles of grape thaumatin-like protein and chitinase in white wine haze formation. J. Agric. Food Chem. 59 (2): 733.
Marangon M., Van Sluyter S.C., Waters E.J. and Menz R.I. 2014. Structure of haze forming proteins in white wines: Vitis vinifera Thaumatin-Like Proteins. PloS One. 9 (12): e113757.
Marchal R., Bouquelet S. and Maujean A. 1996. Purification and partial biochemical characterization of glycoproteins in a champenois Chardonnay wine. J. Agric. Food Chem. 44 (7): 1716.
Monteiro S., Piçarra-Pereira M., Mesquita P., Loureiro V., Teixeira A. and Ferreira R. 2001. The wide diversity of structurally similar wine proteins. J. Agric. Food Chem. 49 (8): 3999.
Monteiro S., Picarra-Pereira M.A., Loureiro V.B., Teixeira A.R. and Ferreira R.B. 2007. The diversity of pathogenesis-related proteins decreases during grape maturation. Phytochemistry. 68 (4): 416.
Pastorello E.A., Farioli L., Pravettoni V., Ortolani C., Fortunato D., Giuffrida M.G., Perono Garoffo L., Calamari A.M., Brenna O. and Conti A. 2003. Identification of grape and wine allergens as an endochitinase 4, a lipid-transfer protein, and a thaumatin. J. Allergy Clin. Immun. 111 (2): 350.
Sauvage F.X., Bach B., Moutounet M. and Vernhet A. 2010. Proteins in white wines: thermo-sensitivity and differential adsorbtion by bentonite. Food Chem. 118 (1): 26.
Segrest J.P. and Jackson R.L. 1972. Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Methods Enzymol. 28: 54.
Shevchenko A., Tomas H., Havlis J., Olsen J.V. and Mann M. 2006. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat. Protoc. 1 (6): 2856.
Siebert K.J., Carrasco A. and Lynn P.Y. 1996. Formation of protein-polyphenol haze in beverages. J. Agric. Food Chem. 44 (8): 1997.
Van Sluyter S.C., McRae J.M., Falconer R.J., Smith P.A., Bacic A., Waters E.J. and Marangon M. 2015. Wine protein haze: mechanisms of formation and advances in prevention. J. Agric. Food Chem. 63 (16): 4020.
Van Sluyter S.C., Marangon M., Stranks S.D., Neilson K.A., Hayasaka Y., Haynes P.A., Menz R.I. and Waters E.J. 2009. Two-step purification of pathogenesis-related proteins from grape juice and crystallization of thaumatin-like proteins. J. Agric. Food Chem. 57 (23): 11376.
Vincenzi S., Polesani M. and Curioni A. 2005. Removal of specific protein components by chitin enhances protein stability in a white wine. Am. J. Enol. Viticult. 56 (3): 246.
Vincenzi S. and Curioni, A. 2005. Anomalous electrophoretic behavior of a chitinase isoform from grape berries and wine in glycol chitin-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. Electrophoresis. 26 (1): 60.
Vincenzi S., Bierma J., Wickramasekara S.I., Curioni A., Gazzola D. and Bakalinsky A.T. 2014. Characterization of a grape class IV chitinase. J. Agric. Food Chem. 62 (24): 5660.
Vincenzi S., Crapisi A. and Curioni A. 2012. Foamability of Prosecco wine: cooperative effects of high molecular weight glycocompounds and wine PR-proteins. Food Hydrocolloid. 34: 202.
Vincenzi S., Marangon M., Tolin S. and Curioni A. 2011. Protein evolution during the early stages of white winemaking and its relations with wine stability. Aust. J. Grape Wine R. 17 (1): 20.
Vincenzi S., Mosconi S., Zoccatelli G., Pellegrina C.D., Veneri G., Chignola R., Peruffo A., Curioni A. and Rizzi C. 2005. Development of a new procedure for protein recovery and quantification in wine. Am. J. Enol. Viticult. 56 (2): 182.
Waters E.J., Alexander G., Muhlack R., Pocock K.F., Colby C., O'Neill B.K., Hoj P.B. and Jones P. 2005. Preventing protein haze in bottled white wine. Aust. J. Grape Wine R. 11 (2): 215.
Waters E.J., Hayasaka Y., Tattersall D.B., Adams K.S. and Williams P.J. 1998. Sequence analysis of grape (Vitis vinifera) berry chitinases that cause haze formation in wines. J. Agric. Food Chem. 46: 4950.
Waters E.J., Shirley N.J. and Williams P.J. 1996. Nuisance proteins of wine are grape pathogenesis-related proteins. J. Agric. Food Chem. 44 (1): 3.
Waters E.J., Wallace W. and Williams P.J. 1992. Identification of heat-unstable wine proteins and their resistance to peptidases. J. Agric. Food Chem. 40 (9): 1514.
Published
2016-08-24
How to Cite
GAZZOLA, D. et al. Characterization of chitinase isoforms from grape juice. Italian Journal of Food Science, [S.l.], v. 29, n. 1, aug. 2016. ISSN 1120-1770. Available at: <http://www.chiriottieditori.it/ojs/index.php/ijfs/article/view/551>. Date accessed: 21 aug. 2018. doi: https://doi.org/10.14674/1120-1770/ijfs.v551.

Keywords

chitinase, electrophoresis, glycol chitin, grape juice, isoform, mass spectrometry